Table 1. Superhelical Densities, Binding Constants, and Single-Turnover Kinetic Constants for Relaxation of MCs by WT vTopo.
| no. of supercoilsa | superhelical densityb | klim′ (M–1 s–1) | KDc (nM) | |
|---|---|---|---|---|
| MCsp | –2.7 ± 1.1 | –0.062 | 1.7 × 106 | NDd |
| MCsp* | –5.4 ± 1.7 | –0.125 | NDd | NDd |
| i–1 | –1e | –0.023 | NDd | NDd |
| MCsp2 | –11.2 ± 1.4 | –0.066 | NDd | NDd |
| pool I1 | –7 ± 1e | –0.042 | NDd | NDd |
| pool I2 | –4 ± 1e | –0.024 | NDd | NDd |
| MCns | –2.7 ± 1.1 | –0.062 | 5.9 × 104 | 0.38 |
| MCns* | –5.4 ± 1.7 | –0.125 | NDd | 0.54 |
| MCnsL | 0 | 0 | NDd | 0.70 |
a
Determined by band counting using chloroquine gel electrophoresis (Figures S1 and S2 of the Supporting Information). Reported as the mean ± the standard deviation of a Gaussian fit.
b
Determined using eq 1.
c
Determined using eq 3. The units are nanomolar minicircle.
d
Not determined.
e
These values were calculated using the most prevalent topoisomer in the intermediate pool, which was then used to calculate the average superhelical density of the pool.