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. 2014 Jun 19;53(26):4302–4315. doi: 10.1021/bi500571q

Table 2. Kinetic Parameters for Supercoil Unwinding of MCs by WT and K271E vTopoa.

  klim (s–1) ks→p/ks→i ki→p/kligc
MCsp 0.029 ± 0.0012 not determined 0.0074 ± 0.0016
MCsp* 0.014 ± 0.0006 1.2 ± 0.12 0.008 ± 0.00088
MCsp2 0.019 ± 0.0004    
  (0.0023 ± 0.000074)b    
pool I1   2.1 ± 0.22 0.0084 ± 0.0013
    (7.7 ± 1.5)b (0.017 ± 0.0094)b
pool I2   3.8 ± 0.26 0.0032 ± 0.00026
    (15.1 ± 5.1)b (0.013 ± 0.017)b
a

Determined using numerical simulations (Supporting Information).

b

Fitted parameters for K271E are shown in parentheses.

c

klig was set to 4 and 0.17 s–1 for WT and K271E vTopo, respectively (Supporting Information).