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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Tissue Eng Regen Med. 2012 May 18;7(11):911–920. doi: 10.1002/term.1483

Figure 2. MSC characterization.

Figure 2

I. mRNA expression of MSC markers. Real Time PCR graph showing negative expression of CD11b and positive expression of CD73, CD90, CD105 and CD106 in second, fourth and sixth passages in isolated MSCs. GAPDH was used as the house keeping gene. The specificity of the primers was analyzed by running a melt curve. II. Multilineage differentiation of MSCs in vitro. A) Alizarin S red staining of MSC culture after 14 days stimulation with osteogenic differentiation medium (DM) B) High magnification image showing osteogenic differentiation C) Oil O Red staining after 21 days of stimulation with adipogenic DM; D) Chondrogenic pellet formed after 21 days of stimulation with chondrogenic DM, E) Safranin O staining of cryosections of chondrogenic pellet, F) Alcian blue staining of cryosections of chondrogenic pellet.