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. 2014 Jul 9;9(7):e100877. doi: 10.1371/journal.pone.0100877

Figure 1. Establishment of the LAMP assay.

Figure 1

A. Different reaction temperature. Lanes: M, DNA marker; 1 to 6, 60 to 65°C; 7, water control. B. Different reaction time. Lanes: M, DNA marker; 1 to 6, 10 to 60 min; 7, water control. C. Amplification of LAMP from positive f-DNA and restriction digestion of LAMP product. Lanes: M, DNA marker; 1, LAMP products from positive f-DNA; 2, water control; 3, EcoR1 digestion of LAMP products. D. Specificity of LAMP assay for E. granulosus (G1 genotype). Lanes: M, DNA marker; 1 and 2, g-DNA and f-DNA of E. granulosus (G1 genotype); 3 to 9, g-DNA of E. multilocularis, E. shiquicus, T. hydatigena, T. multiceps, T. pisiformis, T. taeniaeformis, Dipylidium sp.; 10, intestinal tissues of dogs; 11, n-f-DNA; 12, water control. E. Sensitivity of LAMP assay for DNA from different numbers of E. granulosus eggs per gram of faeces. Lanes: 1, negative control; 2 to 6, one to five eggs; 7, ten eggs; 8, fifteen eggs; M, DL2000.