Table 1.
E. coli strains used in this study
| Strain | Relevant Genotype | Source or Reference |
|---|---|---|
| MG1655 | F- λ − rph- | E. coli Genetic Stock Center |
| MG1655 polA::Zeo |
F- λ − rph- polA_ΔC b | This work |
| MG1655 polA_SD− |
F- λ − rph polA_F769A/F771A ::cat | This work |
| KA797 | polA_D424A ::cat | [22] |
| RW900a | ΔpolA::Kan | LGI stocks |
| KM52 | ΔmutL460::cat | Martin Marinus |
| JW4128 | ΔmutL720::Kan | E. coli Genetic Stock Center |
| ES1484 | mutL218::Tn10 | E. coli Genetic Stock Center |
| JW0178 | ΔrnhB782::Kan | E. coli Genetic Stock Center |
| JW5446 | Δxni725::Kan | E. coli Genetic Stock Center |
| JW0221 | ΔdinB749::Kan | E. coli Genetic Stock Center |
| NR9548 | mutD5 zaf13::Tn10 | [53] |
| EMBL1789 | polA107 | E. coli Genetic Stock Center |
| ET1214 | zih-219::Tn10 | E. coli Genetic Stock Center |
| RW896 | polA107 zih-219::Tn10 | EMBL1789 x P1. ET1214 |
| RW584a | recA730 lexA51(Def) Δ(umuDC)596::ermGT | [5] |
| RW698a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble | [5] |
| RW710a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble mutL215::Tn5 | [34] |
| RW928a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble polA_D424A ::cat | RW698 x P1.KA797 |
| RW1048a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble polA_D424A ::cat ΔmutL720::Kan | RW928 x P1.JW4128 |
| RW932a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble Δxni725::Kan | RW698 x P1 JW5446 |
| RW1050a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble Δxni725c ΔmutL720::Kan | RW932 x P1.JW4128 |
| RW948a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble polA107 zih-219::Tn10 | RW698 x P1. RW896 |
| RW1088a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble polA107 zih-219::Tn10 ΔmutL720::Kan | RW948 x P1. JW4128 |
| RW968a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble polA107 zih-219::Tn10 Δxni725::Kan | RW948 x P1. JW5446 |
| RW1054a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble polA107 zih-219::Tn10 Δxni725::Kan ΔmutL460::cat | RW968 x P1. KM52 |
| RW1030a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble polA_F769A/F771A ::cat | RW698 x P1. MG1655polA_SD− |
| RW1042a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble polA_F769A/F771A ::cat ΔmutL720::Kan | RW1030 x P1. JW4128 |
| RW1060a | recA730 lexA51(Def) Δ(umuDC)596::ermGT polA_ΔC b | RW584 x P1. MG1655polA::Zeo |
| RW1086a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB749::Kan polA_ΔC b | RW1060 x P1. JW0221 |
| RW1098a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB749::Kan polA_ΔC b ΔmutL460::cat | RW1086 x P1. KM52 |
| RW838a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble ΔrnhB782::Kan | [5] |
| RW970a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔrnhB782 ΔdinB61::ble | KanS derivative of RW838c |
| RW1056a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔrnhB782 ΔdinB61::ble ΔmutL720::Kan | RW970 x P1 JW4128 |
| RW1318a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔrnhB782 ΔdinB749::Kan | RW970 x P1. JW0221 |
| RW1326a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔrnhB782 ΔdinB749::Kan polA_ΔC b | RW1318 x MG1655polA::Zeo |
| RW1336a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB749::Kan ΔrnhB782 polA_ΔC b ΔmutL460::cat | RW1326 x KM52 |
| RW1214a | recA730 lexA51(Def) polBex1 Δ(umuDC)596::ermGT ΔdinB749::Kan | LGI lab stocks |
| RW980a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble polA_D424A ::cat ΔrnhB782::Kan | RW928 x P1. JW0178 |
| RW1320 | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble polA_D424A ::cat ΔrnhB782::Kan mutL218::Tn10 | RW980 x P1.ES1418 |
| RW1214a | recA730 lexA51(Def) polBex1 Δ(umuDC)596::ermGT ΔdinB749::Kan | LGI stocks |
| RW1224a | recA730 lexA51(Def) polBex1 Δ(umuDC)596::ermGT ΔdinB749 | KanS derivative of RW1214c |
| RW1226a | recA730 lexA51(Def) polBex1 Δ(umuDC)596::ermGT ΔdinB749 ΔmutL720::Kan | RW1224 x P1.JW4128 |
| RW1242a | recA730 lexA51(Def) polBex1 Δ(umuDC)596::ermGT ΔdinB749 polA_ΔC b | RW1224 x MG1655polA::Zeo |
| RW1324a | recA730 lexA51(Def) polBex1 Δ(umuDC)596::ermGT ΔdinB749 polA_ΔC b ΔmutL460::cat | RW1242 x KM52 |
| RW1218a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB749::Kan polA_ΔC b ΔmutL460::cat mutD5 zaf13::Tn10 | RW1098 x P1. NR9548 |
| RW1250a | recA730 lexA51(Def) Δ(umuDC)596::ermGT ΔdinB61::ble ΔmutL720::Kan mutD5 zaf13::Tn10 | RW1236 x P1. NR9548 |
a
: thr-1 araD139 Δ(gpt-proA)62 lacY1 tsx-33 glnV44 galK2 hisG4 rpsL31 xyl-5 mtl-1 argE3 thi-1 sulA211
b
: encodes polA residues 1–768 and expresses 5′→3′ exo/endonuclease and 3′→5′ exonuclease functions of pol I.
c
: The KanR cassette was “Flp’d” out by transforming the strain with the temperature sensitive plasmid, pCP20 encoding the FLP recombinase, followed by overnight incubation at 43°C [54].