Table 2.

Spectrum of spontaneous mutations generated in the rpoB gene of recA730 lexA(Def) ΔdinBΔ umuDCΔ mutLrnhB ⁺/ΔrnhB polA_D424A and polA_ΔC strains in the presence of umuC_Y11A^a.

	Position b	bp change	rnhB+ polA_D424A	ΔrnhB polA_D424A	rnhB+ polA_ΔC	ΔrnhB polA_ΔC
	1532	AT→GC	29	19	10	9
	1534	AT→GC	72	96	1	32
	1535	CG→AT				1
	1535	CG→TA	10	2		2
	1537	CG→AT	1
	1538	AT→TA		1		1
	1538	AT→GC	5	6		15
	1546	CG→AT		3		1
	1546	CG→TA	18	64	34	30
	1547	AT→TA	1	9		23
	1547	AT→GC	90	10	242	32
	1552	AT→GC	2	2	1	1
	1565	CG→TA				1
	1575	CG→GC			1
	1576	CG→AT	13	6		19
	1576	CG→TA	5	11		91
	1577	AT→TA		9		6
	1586	CG→TA				1
	1592	CG→AT		3
	1592	CG→TA	4	2		6
	1593	CG→TA				1
	1598	AT→TA		1
	1598	AT→GC	3	6		2
	1600	CG→TA		2
	1611	CG→TA		1
	1691	CG→TA	5			6
	1695	AT→GC			1
	1708	CG→TA			1
	1711	CG→TA		1
	1714	AT→TA	25	34		6
		Transitions	243 (86%)	221 (77%)	290 (99%)	229 (80%)
		Transversions	40 (14%)	67 (23%)	1 (0.3%)	57 (20%)
		Total	283	288	291	286

^a: The data are the number of mutants found for each type of base substitution at a particular position

^b: The numbering system originates from Garibyan et al., [32], where the A of the ATG initiation codon is #1.