. Author manuscript; available in PMC: 2015 Jun 15.

Published in final edited form as: Cancer Res. 2014 Apr 16;74(12):3271–3281. doi: 10.1158/0008-5472.CAN-13-2015

Table 1.

Characterization and physicochemical properties of PLA-PEG block copolymers and nanoparticles

Sample	GPC of copolymers^a			Hydrodynamic diameter^b, nm	Zeta Potential (ζ)	Peptide/polymer ratio^c (w/w)	EE^d%	Particle Size after peptide loading^b, nm	Zeta Potential after peptide loading (ζ)
Sample	Mn	Mw	Mw/Mn	Hydrodynamic diameter^b, nm	Zeta Potential (ζ)	Peptide/polymer ratio^c (w/w)	EE^d%	Particle Size after peptide loading^b, nm	Zeta Potential after peptide loading (ζ)
PLA	59745	72487	1.213	125±3.7	−15.8±1.45	1:10	42.93	132±2.3	−30.07±2.56
PLA-PEG^1K	67916	72806	1.072	120±2.5	−10.2±2.04	1:10	52.09	115±3.2	−25.26±4.01
PLA-PEG^2K	70692	73485	1.040	117±3.9	−4.7±1.09	1:10	58.96	119±2.7	−20.74±3.10
PLA-PEG^4K	72479	78416	1.082	114±1.9	−3.3±0.95	1:10	65.55	111±4.6	−22.90±1.54
PLA-PEG-PPG-PEG^12-5K	80256	85678	1.066	120±2.3	−3.1±1.23	1:10	64.04	118±2.5	−21.14±2.45
PLA^12K-PEG-PPG-PEG^125K	11234	12676	1.182	106±2.7	N.D.	1.:10	56.12	N.D.	N.D.

Gel permeation chromatography (GPC) of PLA-PEG block copolymers at room temperature using Viscoteck GPC system with THF as mobile phase

Measured by NTA (Nanoparticle Tracking Analysis; NS500, Nanosight)

Concentration of the polymer was taken as 20 mg/ml

Encapsulation efficiency expressed as a percentage mean of 3 determinants ± S.D. of NuBCP-9 recovered in NPs compared with theoretical load

N.D. Not done