Skip to main content
. Author manuscript; available in PMC: 2014 Jul 9.
Published in final edited form as: J Opt Soc Am A Opt Image Sci Vis. 2013 Aug 1;30(8):1640–1645. doi: 10.1364/JOSAA.30.001640

Table 1. Typical Values of χ for Different STED Systemsa.

Dye Wavelengths (nm)
Pulsed-STED
χ 		CW-STED
χ
Excitation Depletion
ATTO 532 470 603 0.31 0.40
Alexa Fluor 488, Chromeo 488, 488 592 0.36 0.45
FITC, DY-495, Citrine GFP 490 575 0.42 0.53
Citrine, YFP 490 600 0.35 0.44
Pyridine 2, RH414 554 745 0.26 0.33
Alexa 594, Daylight 594, ATTO 594 570 690 0.36 0.45
ATTO 633, ATTO 647N, Abberior STAR635 635 750 0.42 0.52
a

Dates of excitation and depletion wavelengths are from [30]. Numerical aperture of the lens is set to be NA = 1.4, and α = 2.15 for pulsed-STED, α = 1.82 for CW-STED.