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. 2014 Jul 9;9(7):e98209. doi: 10.1371/journal.pone.0098209

Table 1. PIV data acquisition and analysis parameters.

Laser & illumination 5.5 W, 532-nm continuous wave Nd∶YAG,
Time interval: min. 110 µs (70% stenosed), max. 400 µs (normal model)
Illumination width: min. 100 µs
Camera & acquisition 1024×1024 pixels, 10-bit CMOS
Sensor pitch size: 17 µm
Acquisition rate: 1 kHz (500 Hz double frame)
Image properties Viewing angle: ±35°
Lens focal length: 60 mm
Lens focal aperture: 2.8
Filters: 550-nm long-pass
Field of view: ∼45×41 mm2
Magnification: ∼38 µm per pixel
Image pre-processing Sliding background subtraction
RMS mask to eliminate non-flow regions
Particles & image density 15-µm Rhodamine-B infused polystyrene 2.5 pixels mean
2.5 pixels mean image particle size
Average of ∼9 particles per 16×16 px2 window
Vector analysis No. of measurement planes per model: ∼15 No. of velocity
No. of velocity vectors per model: ∼21,000
Type: adaptive multi-pass processing with decreasing window size from 64×64 to 16×16, using 3 refinement passes
Final grid spacing: 8 pixels (∼0.3 mm)
Vector validation Median filter (remove and replace)
Single pass smoothing (3×3)