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. Author manuscript; available in PMC: 2014 Jul 9.
Published in final edited form as: Biochem J. 2011 Feb 15;434(1):83–92. doi: 10.1042/BJ20101358

Figure 4. Changes in p53, p21 and c-Myc protein levels after induction of hOsa2.

Figure 4

(A) 16eC3 cells were treated with Dox for the indicated hours and then were harvested and fractionated into NE (nuclear) and PNS fractions. Proteins were separated by SDS/PAGE and immunoblotted with antibodies against hOsa2, p53, p21, or c-Myc. Molecular size markers are indicated at left. (B) Lysates of 16eC3 cells induced for 24 h (+) were separated by SDS/PAGE and probed for total p53, p53 phospho-Serine 15, and p53 phospho-Serine 37. *Non-specific cross-reacting protein.