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. 2014 Jul 9;9(7):e101774. doi: 10.1371/journal.pone.0101774

Figure 1. NE increased EPCs in peripheral blood in mice with limb ischemia.

Figure 1

Cells from peripheral blood were lysed and analyzed by flow cytometry. Cells were sequentially gated based on CD45 (A), CD34 and KDR expression (C). Circulating EPCs were defined as CD45-/CD34+/KDR+ cells. A gate was used to select the total CD45- cell population (A). Corresponding flow cytometric analysis was used to detect CD34+/KDR+ cells in the gated CD45- cell population. Proportion of EPCs in peripheral blood was increased from 0.15±0.04% to 0.31±0.05% after intraperitoneal injection of NE in limb ischemia model (B, * P<0.05 compared with the model group). Representative flow cytometric analysis of EPCs (CD34/Flk-1 cells) were showed in part C.