Table 2. Survey of the most abundant molecular species of A. castellanii phospholipids determined by LC/MS operating in the negative (−) and positive (+) ion mode.

PL class	m/z	Fatty acyl composition
LPC	480.2 (+)	16∶0
	506.2 (+)	18∶1
	508.3 (+)	18∶0
PC	750.8 (−)a	16∶0/14∶0
	758.6 (+)	16∶1/18∶1c
	760.6 (+)	16∶0/18∶1c
	764.8 (−)	17∶0/14∶0c
	778.8 (−)	14∶0/18∶0
	784.6 (+)	18∶1/18∶2
	786.6 (+)	18∶1/18∶1
	788.6 (+)	18∶0/18∶1
	806.6 (+)	18∶1/20∶5c
	812.6 (+)	18∶1/20∶2
	816.4 (−)	14∶0/20∶2; 16∶1/18∶1
	846.4 (−)	18∶0/18∶1
	850.8 (−)	18∶2/20∶4
	852.8 (−)	18∶1/20∶4
	854.8 (−)	18∶0/20∶4
	854.7 (−)	18∶1/20∶3
	856.8 (−)	18∶0/20∶3
	858.9 (−)	18∶0/20∶2
	866.5 (−)	18∶2/20∶3; 18∶1/20∶4
	868.7 (−)	18∶0/20∶4c
PE	688.4 (−)	14∶0/18∶1
	714.5 (−)	16∶1/18∶1; 16∶0/18∶2; 14∶0/20∶2
	716.4 (−)	16∶0/18∶1
	742.5 (−)	16∶0/20∶2; 18∶0/18∶2; 18∶1/18∶1
	744.5 (−)	18∶1/18∶0
	764.8 (−)	18∶1/20∶4; 18∶2/20∶3
	766.8 (−)	18∶2/20∶2; 18∶1/20∶3; 18∶0/20∶4
	768.8 (−)	18∶0/20∶3; 18∶1/20∶2
	768.7 (+)	18∶0/20∶4
	882.6 (−)	30∶2/16∶0; 28∶1/18∶1 b
	908.6 (−)	28∶1/20∶2; 30∶2/18∶1
	910.6 (−)	30∶1/18∶1; 28∶0/20∶2
	930.6 (−)	30∶2/20∶4
	932.9 (+)	30∶3/20∶3; 20∶4/30∶2
	934.6 (−)	30∶2/20∶2
	938.5 (+)	20∶1/30∶2
PS	758.7 (+)	16∶1/18∶1c
	804.6 (−)	18∶2/20∶5
	806.4 (−)	18∶2/20∶4; 18∶1/20∶5
	808.5 (−)	18∶1/20∶4
	810.7 (−)	18∶1/20∶3c
	812.9 (−)	18∶0/20∶3
	812.6 (+)	18∶1/20∶2c
	972.6 (−)	30∶3/20∶4
	978.6 (−)	30∶2/20∶2
PI	831.5 (−)	16∶1/18∶2
	833.5 (−)	16∶1/18∶1
	835.5 (−)	16∶0/18∶1
	847.4 (−)	17∶0/18∶2
	859.5 (−)	18∶1/18∶2
	863.6 (−)	18∶1/18∶0
	889.6 (−)	18∶0/20∶2
PA	645.2 (−)	16∶0/16∶1
	661.5 (−)	15∶0/18∶1
	673.5 (−)	16∶0/18∶1
	675.5 (−)	16∶0/18∶0
	689.9 (−)	18∶0/17∶0c
	695.5 (−)	18∶1/18∶2
	865.6 (−)	18∶1/30∶2 c
	865.6 (−)	28∶1/20∶2 c

^aPhosphatidylcholines were detected as acetate [M+CH₃COO]⁻ or formate [M+HCOO]⁻ adducts.

^bbold-print - PL with long chain FAs.

^cPL species without assignments to sn-1/sn-2 position.