Figure 4. Functional synergy and crosstalk between MPRNs activated by different targeted manipulations.

(A) Functional synergy and crosstalk between MPRNs activated by three miRNA targeted manipulations. Since miR-181d was upregulated by all the three manipulations, its target pathways and fold changes are provided. Green circles and blue triangles represent functional miRNAs and targeted pathways, respectively. (B) Functional synergy and crosstalk between MPRNs activated by three TF targeted manipulations. Since eight miRNAs were shared by all the three manipulations, their fold changes under all three manipulations are shown. (C) Example of functional synergy between MPRNs associated with manipulations targeting miRNAs and TFs. Since protein processing in the endoplasmic reticulum pathway was shared by +miR-181d, −miR-23b, −β-catenin, and −STAT3, miRNAs regulating this pathway are shown. The distribution of mRNAs with altered expression in this pathway is shown in the diagram, where purple, cyan, and red rectangles represent mRNAs deregulated by manipulations targeting miRNA, TFs, or both, respectively. HSP90B1 (circled in green) was downregulated by all the four experimental conditions.