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. 2014 Jul 9;9(7):e102238. doi: 10.1371/journal.pone.0102238

Figure 6. Rhythmic transcription of genes with CRBSs in their promoter is masked by high baseline expression.

Figure 6

Robust low amplitude transcription rhythms revealed by long-term luminescence measurements of stable U2OS cell lines expressing the destabilized luciferase2P (luc2P) under control of the promoters of ATG3, EIF5A2, and SCN5A. BMAL1-luc, PER2-luc2, and GAPDH-luc2P cell lines are shown for control. Luminescence of synchronized cultures was measured at 30 min intervals. Raw expression data of the indicated reporter genes for day 1 to day 5 after synchronization are shown in the left panels. The middle panels show the 24 h moving average of the luciferase activity to estimate the non-rhythmic contribution and the right panels show the de-trended data to estimate the rhythmic contribution. The black and grey traces show expression profiles in the absence and presence of 200 nM rapamycin, respectively.