Figure 2. Modulation of the eye defects of N^spl by Wdb.

Scanning EM of the adult eye at a magnification of 200x (A–D) and 1000x (B’–D’). (A) wdb^KG02977 homozygous flies display wild type eyes, whereas those of N^spl/Y (males) are reduced and rough (B, B’). The N^spl eye is exacerbated by wdb^KG02977 (C, C’), but rescued by overexpression of UAS-wdb (D, D’). Graph shows ommatidial (facet) counts of the eyes in panels B–D. Facet counts were determined in ≥15 flies, and asterisks denote P-values<0.001. (E) Facet counts in other relevant genotypes; note that wild type flies typically display 750–800 facets. Inset shows R8 selection from intermediate groups (IG). During R8 specification, Ato expression initiates at stage-1 of the MF, then upregulates, and finally resolves at stage-2/3, a region that coincides with the expression domain of 109-68Gal4. Color codes are; Ato (pink/red), Sens (blue) and secondary R cells (grey).