Figure 4. Ectopic Wdb rescues the eye defects of N^spl/+; E(spl)D/+ flies.

Scanning EM of the adult eye at 200x magnification (A–C). (A) Overexpression of wdb does not perturb the adult eye. The reduced eye of N^spl/+; E(spl)D/+ flies (B) is rescued by overexpression of Wdb (C). Eye discs of the genotypes indicated in A–C were stained with α-Sens (Green) and α-ELAV (Red) to assess R8 differentiation and secondary photoreceptor recruitment, respectively, and arrows denote direction of MF progression. Panels A’–C’ show Sens+ELAV staining, whereas A’’–C’’ show Sens-only channel to highlight differentiated R8s. Graph shows ommatidial (facet) counts of the adult eyes. Data labeled B and C correspond to the adult eye shown in panels B and C. The genotype of relevant control progeny (D, E) is noted. Facet counts were determined in ≥15 flies, and asterisk denotes P-values<0.001.