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. 2014 Jul 9;9(7):e101884. doi: 10.1371/journal.pone.0101884

Figure 6. Ectopic Wdb rescues the eye defects of the CK2 mimic M8-S159D.

Figure 6

Scanning EM of the adult eye at 200x magnification (A–B). (A) Overexpression of M8-S159D elicits a moderately reduced eye (A), which is strongly rescued by co-expression of Wdb. Graph shows ommatidial (facet) counts of the adult eyes. Data labeled A and B correspond to the adult eye shown in panels A and B. The genotype of relevant control progeny (C) is noted. Facet counts were determined in ≥15 flies, and asterisk denotes P-values<0.001. Eye discs of the genotypes indicated in A–B were stained with α-Sens (Green) and α-ELAV (Red) to assess R8 differentiation and secondary photoreceptor recruitment, respectively, and arrows denote direction of MF progression. Panels A’–B’ show Sens+ELAV staining, whereas B’’ show a magnification of B’ to highlight rescue of differentiated R8s and ELAV clusters.