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. Author manuscript; available in PMC: 2014 Jul 9.
Published in final edited form as: Biotechniques. 2014 Feb 1;56(2):90–94. doi: 10.2144/000114137

Table 1.

Antibodies tested for ability to stain mitotic cells.

Antibody Abbreviation Species, Clonality Source, Catalog number Epitope description
Anti-phospho Histone H3 (Ser10), Mitosis Marker H3S10ph Rabbit, polyclonal EMD Millipore, 06-570 and 04-817 Phosphorylated serine 10 on the N-terminal tail of histone H3; occurs during late G2 phase to anaphase (12)
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) MPM2 Mouse, monoclonal EMD Millipore, 05-368 Phosphorylated serines or threonines followed by proline, specifically peptides LpTPLK or FpTPLQ; occurs during mitosis (9,13)
Alexa Fluor 647 Rat anti-Histone H3 (pS28) H3S28ph Rat, monoclonal BD Biosciences, 558217 Phosphorylated serine 28 on the N-terminal tail of histone H3; occurs during prophase to anaphase (10)
Anti-HpTGEKP motif HpTGEKP Rabbit, polyclonal EMD Millipore, ABE319 Peptide HpTGEKP; based on the highly conserved linker joining adjacent C2H2 zinc fingers; occurs during prophase to anaphase (11)