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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2014 Apr 18;13(7):1826–1836. doi: 10.1158/1535-7163.MCT-13-1093

Fig. 3. The CM combination inhibits HNSCC cell growth in vitro.

Fig. 3

A, for colony formation assay, SCC47 and Tu212 cells at 200/well were treated with 20μg/mL MM-121, 2μg/mL cetuximab or the combination for 9 days. The medium was changed every three days before cells were stained with 0.2% crystal violet. Cell numbers ≥50 were considered as a colony. ImageJ software was used for colony counting. B, cells (2.5 × 105/well) were cultured for overnight and treated with MM-121 125μg/ml, cetuximab 62μg/ml or the combination for 72 hours and then trypsinized and counted. Error bars are mean ± SE from 3 independent experiments. (* indicates p<0.05 vs. ctrl, ** indicates p<0.05 vs. all other groups).