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. Author manuscript; available in PMC: 2015 Jul 7.
Published in final edited form as: Curr Biol. 2014 Jun 19;24(13):1518–1524. doi: 10.1016/j.cub.2014.05.029

Figure 3.

Figure 3

The CRT and EE in the LUX promoter confer evening-phased oscillations in the cold

Bioluminescence assays in Arabidopsis seedlings carrying luciferase reporters driven by LUX promoter fragments (A–C) or multimerized CRT and EE motifs (D and E). Ten-day old seedlings were entrained under 12:12 at 22°C. Seedlings remained under these conditions (B and D) or were transferred to constant light at 22°C (A) or 12:12 at 4°C (C and E). Red and blue bars represent 22°C and 4°C respectively. Seedlings in the T1 (A,D,E) or T2 (B,C) generation were assayed. Data shown is the averages of all plants imaged in a given experiment with error bars representing standard error. Values in B and C are relative to the average expression of that reporter line. Traces are representative of data from at least two independent experiments.