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. 2014 Jun 25;25(2):37–43. doi: 10.1294/jes.25.37

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2014 The Japanese Society of Equine Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 3. — DGGE analysis of the PCR products of lactic acid bacteria present in the neonatal Thoroughbred feces by lactic acid bacteria-specific primers [25]. Approximately 200 bp 16S rDNA of E. coli No. 341–534 were amplified by PCR. Lane 1: meconium, lane 2: feces obtained on the 7th day after birth, lane 3: feces obtained on the 14th day after birth, lane 4: feces obtained on the 21st day after birth. Band a: L. johnsonii (100% similarity), band b: L. equi (100% similarity), band c: L. ruminis (99.9% similarity), and band d: L. reuteri (99.8% similarity).