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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Biochemistry. 2013 Mar 1;52(10):1802–1813. doi: 10.1021/bi3016668

Fig 3.

Fig 3

ELISA analysis of the specificity of selected b12 reactive phage clones. The indicated b12 antibodies (wild-type or mutant; top axis) were immobilized in triplicate in plate wells, and phage clones displaying the indicated αIMs (left axis) were then added to the wells. After washing, bound phage was detected using anti-M13-HRP conjugate. B2.1 is a positive control phage encoding a peptide mimotope that binds wild type b12 MAb as well as the three mutant forms of the b12 MAb [9, 18]. CRPII is an irrelevant phage clone, not selected against the b12 MAb.