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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Biochemistry. 2013 Mar 1;52(10):1802–1813. doi: 10.1021/bi3016668

Fig 7.

Fig 7

The 2F5 and 4E10 αIMs competitively inhibit the binding of their cognate antibodies to their target epitopes in HIV-1 Env. (A) 2F5 epitope peptide conjugated to BSA was immobilized in triplicate wells. A fixed concentration of 2F5 MAb was then pre-incubated with half log dilutions of 2F5 αIM, MPER peptide, or an irrelevant αIM (F10λ) before addition to the coated plate. After washing, bound 2F5 Mab was detected using anti-human IgG-HRP. (B) 4E10 epitope peptide conjugated to BSA was immobilized in triplicate wells. A fixed concentration of 4E10 was then pre-incubated with half log dilutions of 4E10 αIM (5-5 or 5-2), MPER peptide, or an irrelevant αIM (F10λ) before addition to the coated plate. After washing, bound 4E10 Mab was detected using anti-human IgG-HRP. (C, D) Sera from an HIV-positive person (026) (C), or from an HIV-negative person (1609) (D) was pre-incubated with half log dilutions of 4E10 5-2 αIM, 4E10 5-5 αIM, MPER peptide, or F10λ αIM before addition to a plate coated with 4E10 epitope peptide. After washing, bound 4E10 MAb detected with anti-human IgG-HRP.