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Rtx αIM can be used as a sensitive probe to detect Rituximab in normal human sera. Rtx αIM or b12 3–5 αIM was immobilized in wells in triplicate. Rituximab was then serially diluted in 1% pooled normal human sera (itself diluted in blocking solution), and added to the wells. After extensive washing, bound Rituximab was detected with anti-human IgG-HRP.
