Figure 6.

Properties of CMCase produced by the strain ME27-1. (a) Effect of pH on CMCase activity. (b) Effect of temperature on CMCase activity. (c) Effect of pH on the stability of CMCase. (d) Thermostability of CMCase. The different buffers used are as follows (100 mM): sodium citrate buffer (empty square; pH 3.0–6.5), Na₂HPO₄-NaH₂PO₄ buffer (filled square; pH 6.5–7.5), Tris-HCl buffer (empty triangle; pH 7.5–8.5), and glycine-NaOH buffer (filled triangle; pH 8.5–11.0). Error bars show the standard deviation of experimental point (n = 2).