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. 2014 Jul 10;4:5651. doi: 10.1038/srep05651

Figure 1. Effects of andrographolide on cell apoptosis in rat VSMCs.

Figure 1

(a) Cells were treated with andrographolide at indicated concentrations for 24 h or 48 h, and cell viability was then determined by MTT assay. Each column represents the mean ± S.E.M. of at least four independent experiments. (**, P < 0.01, and ***, P < 0.001, compared with the vehicle-treated control group). (b) Photomicrographs showing the effect of treatment with vehicle (i) or 20 μM (ii) or 50 μM (iii) andrographolide for 48 h. (magnification × 100). (c) Cells were treated with andrographolide at indicated concentrations for 48 h. Cells were then stained with annexin V and propidium iodide for 15 min. The percentage of apoptotic cells was then analyzed by flow-cytometric analysis. Results shown are representative of four independent experiments.