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. 2014 Jul 10;4:5651. doi: 10.1038/srep05651

Figure 4. PP2A is involved in andrographolide-induced p38MAPK, p53, and caspase-3 activation in rat VSMCs.

Figure 4

Cells were transiently transfected with pp2a siRNA for 48 h followed by the treatment with andrographolide for another 10 min (a and b) or 48 h (c). The extent of p38 phosphorylation (a), p53 phosphorylation (b) and cleavaged caspase-3 (c) were then examined by immunoblotting. Compiled results are shown at the bottom of the chart. Each column represents the mean ± S.E.M. of at least three independent experiments. The full-length blots are presented in Supplementary Figure 3a, 3b and 3c. (d) Cells were transiently transfected with pp2a siRNA for 48 h followed by the treatment with andrographolide for another 48 h. Cell viability was then determined by MTT assay. Each column represents the mean ± S.E.M. of four independent experiments. (e) Cells were transiently transfected with pp2a siRNA for 48 h followed by the treatment with andrographolide for another 10 min. The extent of PP2A catalytic subunit (PP2A-C) was then examined by immunoblotting. Compiled results are shown at the bottom of the chart. Each column represents the mean ± S.E.M. of three independent experiments. The full-length blots are presented in Supplementary Figure 3d. (*, P < 0.05, and ***, P < 0.001, compared with the control group; and #, P < 0.05, ##, P < 0.01, and ###, P < 0.001, compared with the group treated with andrographolide alone). The full-length blots are presented in Supplementary Figure 3d.