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. 2014 Jul 10;5:339. doi: 10.3389/fmicb.2014.00339

Figure 4.

Figure 4

Assessment of SpyCEP cleavage and hyaluronic acid content. (A) SpyCEP-mediated cleavage of proteins involved in bacteria-host cells interactions was assessed. After overnight incubation in the presence of recombinant SpyCEP (rSpyCEP, 100 ng/ml), the samples were loaded on a polyacrylamide gel and cleavage assessed by coomassie blue staining. IL-8 was used as a positive control for the cleavage reaction. (B) β-1 integrin expression on EA.hy926 cells surface after exposure to buffer only (control), rSpyCEP (10 μg/ml) or an unrelated protein (S1LG, 10 μg/ml) purified using the same method. The graph represents two pooled experiments and error bars represent the standard deviation. (C) Hyaluronic acid (HA) content quantification in the GAS strains. Error bars represent the standard deviation, statistical analysis was carried out using a two-tailed t-test (ns = p > 0.05). The p-values are relative to GAS WT.