Figure 4.

Three fragment hits against DC-SIGN. Fragments 1, 2, and 3 were identified during the primary ¹⁹F NMR screening and later confirmed in an SPR follow-up assay. These fragments are similar to substructures of a previously published micromolar DC-SIGN inhibitor 4 (41). Affinities measured in the presence of EDTA and Ca²⁺ are highlighted in black and red, respectively. No binding (n.b.) was detected in presence of EDTA for fragments 1 and 3. SPR measurements were run in triplicates for each condition.