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High-mobility group box 1 protein (HMGB1) expression and the amount of released HMGB1 from four groups of islets. Four groups of islets were stained with insulin (green), HMGB1 (red), and DAPI (blue) (A). HMGB1 mRNA levels were analyzed by qRT-PCR (B). The amount of released HMGB1 was measured by ELISA (C). Scar bars: 50 μm. *p < 0.05.
