Table 2. Number of QD605-labelled import sites per mitochondrion and the percentage found in clusters.
Surface area (μm2) | QDs | QDs as clusters (%) | QDs per μm2 |
---|---|---|---|
0.78 | 47 | 82.6 | 60.5 |
0.79* | 29 | 51.9 | 36.7 |
1.00 | 77 | 57.1 | 76.7 |
1.04 | 73 | 51.6 | 70.5 |
1.14 | 53 | 55.8 | 46.5 |
1.19* | 84 | 72.3 | 70.8 |
1.28* | 116 | 85.2 | 90.7 |
1.46 | 111 | 72.1 | 76.0 |
1.55 | 106 | 76.2 | 68.3 |
1.65 | 97 | 54.2 | 58.9 |
2.02* | 204 | 80.3 | 100.9 |
2.10 | 158 | 61.8 | 75.3 |
Mean | 66.8±12.7 | 69.3±17.5 |
n=12 mitochondria from 12 different tomograms. Mean is given as±s.d.
*Denotes experiments with a different b2Δ-DHFRbiotin and mitochondrial preparation, included to demonstrate reproducibility of results.