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. 2014 May 29;111(1):132–138. doi: 10.1038/bjc.2014.275

Figure 2.

Figure 2

β-catenin deletion diminishes hepatoma cell proliferation. (A) BrdU-labelling index of GS-positive and -negative subpopulations in tumours of Ctnnb1 KO mice after tamoxifen administration. The labelling index is expressed as the percentage of BrdU-positive nuclei in a whole tumour section. On average, 759 nuclei per tumour were counted. Tumours double stained for BrdU and GS are stratified into two groups according to their level of Ctnnb1 knockout as determined by the percentage of GS-negative tumour cells (25–50% KO, n=9; >50% KO, n=13). GS-positive tumour subpopulations show a higher proliferation index compared with the GS-negative ones within the same tumour. (B) Tumour volume fraction from Ctnnb1 KO and WT mice 1 and 7 weeks after tamoxifen application. Livers from WT mice show an increase in tumour burden over time, whereas the tumour volume fraction in livers from Ctnnb1 KO mice is not significantly altered during the 6 weeks' time period. Group sizes: n=8 (WT groups; Ctnnb1 KO 7 weeks); and n=7 (Ctnnb1 KO 1 week). Statistical differences are indicated by one (*P<0.05) or three (***P<0.001) asterisks.