Fig. 3.
Effects of 3-methyladenine [3-MA, (2 × 10−3 mol/L)] or bafilomycin A1 [Baf, (10−4 mol/L)] on Ang II (10−7 mol/L)-induced increase in LC3-II, beclin-1 and decrease in SQSMT1/p62 expressions. Effects of 3-MA (a) and Baf (d) on Ang II-induced increase in the LC3-II to LC3-I ratio, top representative blots of LC3-II and LC3-I expression after treatment with Ang II, bottom densitometry analysis of the ratio of LC3-II to LC3-I. Effects of 3-MA (b) and Baf (e) on Ang II-induced increase in beclin-1 expression, top representative blots of beclin-1 and GAPDH after treatment with Ang II, bottom densitometry analysis of beclin-1 and GAPDH. Effects of 3-MA (c) and Baf (f) on Ang II-induced decrease in SQSMT1/p62 expression, top representative blots of SQSMT1/p62 and GAPDH after treatment with Ang II, bottom: densitometry analysis of SQSMT1/p62 and GAPDH. Control group is expressed as 1. Data are presented as mean ± SEM (n = 6–8). *p < 0.05 vs. control. † p < 0.05 vs. Ang II