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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Dev Dyn. 2013 Sep 2;242(10):1202–1209. doi: 10.1002/dvdy.24017

Figure 1. Inverted microscope guided laser-ablation.

Figure 1

A–C) The laser, conjugated to a 20X objective on an inverted microscope (A) is viewed from above (B) and below the tissue culture dish (C). D–E) Because precise and efficient ablation occurs at the inner surface of the tissue culture plastic, the buoyant embryo is positioned with a silicone rubber holder (D) and further stabilized with a silicone plug (small block in E). F) Correct positioning (foregut facing the bottom of the dish) is verified at higher magnification. G) The field of view during manipulation allows for clear identification of the notochord (No), somites (S) and ventral foregut lip (VF). The white asterisk is placed on the ectoplacental cone in D–F.