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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Methods Cell Biol. 2009 Dec 4;93:121–143. doi: 10.1016/S0091-679X(08)93007-7

Table III.

The Phases of Intraflagellar Transport

Phase of IFT Predicted phenotype of phase-defective mutants
I: Particles bind a cargo and change in structure and/or size in a position close to the basal bodies. Defects in subunits of the particles or basal bodies. Accumulation of particles at proximal end of flagella.
II: Particles and cargo are transported to the distal end of flagella by an anterograde motor. Defects in subunits of the particles or kinesin-2. Defective anterograde velocity of IFT particles. Accumulation of particles at proximal end of flagella.
III: Particles release the cargo and change their structure and/or size at the distal end of the flagella. Defects in subunits of the particles or components of the axoneme or matrix at the flagellar tip. Accumulation of particles at the distal end of flagella.
IV: Particles are transported to a region close to the basal bodies by a retrograde motor. Defects in subunits of the particles or cytoplasmic dynein. Defective retrograde velocity of IFT particles. Accumulation of particles at distal end or along flagella.