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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Nature. 2013 Jun 19;498(7455):497–501. doi: 10.1038/nature12322

Figure 4. Notch signaling is required for zebrafish cardiac regeneration.

Figure 4

(a–d) 30 hpt Tg(vmhc:mCherry-NTR;tp1:eGFP) (a) control; (b) ablated; (c) DAPT-treated control; (d) DAPT-treated ablated hearts. (e, f) Atrium of 30 hpt Tg(vmhc:mCherry-NTR;tp1:eGFP;kdrl:mCherry) (e) control and (f) ablated hearts. Arrow - Endocardial tp1:eGFP expression. (g) DAPT-treated 96 hpt Tg(vmhc:mCherry-NTR;amhc:CreERT2;β-act2:RSG) ablated ventricles. Dashed line - atrioventricular boundary. (h) Recovery of 96 hpt control and DAPT-treated Tg(vmhc:mCherry-NTR;amhc:CreERT2;β-act2:RSG) ablated fish. (i–l) notch1b and (m–p) deltaD in situ hybridization on (i, k, m, o) control or (j, l, n, p) ablated Tg(vmhc:mCherry-NTR) hearts (dashed lines) at 24 hpt. (k, l, o, p) DAPT-treated. A - atrium; AVC - atrioventricular canal; OFT - outflow tract; V - ventricle.