Figure 2. MERO-GFP is fully oxidized under normal conditions and reduced by DTT in vivo.
(A) Histogram of INS-1 832/13 cells stably expressing MERO-GFP treated with DTT. The x-axis indicates the MERO-GFP ratio and the y-axis indicates the number of cells. All error bars shown, ± s.d. (B) The median MERO-GFP ratio of the data shown in Figure 2A. (C) Histogram of MERO-GFP ratio of untreated cells or cells treated with 1 mM H2O2 or 1 mM DTT. (D) Histogram of cytosolic roGFP (cyto-roGFP) ratio of untreated cells or cells treated with 1 mM H2O2 or 1 mM DTT. (E) The median of MERO-GFP or cytosolic roGFP ratio of untreated cells or cells treated with 1mM H2O2 or 1 mM DTT.