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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Lab Invest. 2013 Sep 16;93(11):1254–1258. doi: 10.1038/labinvest.2013.112

Fig. 3. MERO-GFP monitors the dynamic ER redox state in mammalian cells.

Fig. 3

(A) The MERO-GFP ratio in HEK293a cells transduced with control shRNA or shRNA directed against ERO1α and then treated with different concentrations of DTT. (B) The MERO-GFP ratio in HEK293a cells transduced with control shRNA, shRNA directed against ERO1α, or shRNA directed against PRDX4, and then treated with different concentrations of DTT. (C) The MERO-GFP ratio of HEK293a cells transduced with control shRNA, shRNA directed against ERO1α or shRNA directed against PRDX4, and treated with or without 0.2 mM DTT. (D) Expression levels of ERO1α and PRDX4 mRNA in HEK293a cells transduced with control shRNA or shRNA directed against ERO1α (left panel) or PRDX4 (right panel). Expression levels of mRNA were measured by real-time PCR. (E) The oxidizing capacity of the ER in different cell lines. All error bars shown, ± s.d. * p<0.05, **p<0.01.

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