Figure 4. Effect of phospholipase inhibitors on total endogenous SA levels in C. chinense suspension cells. Cells were treated with the PLC inhibitors 100 μM neomycin (N), 10 μM U73122, or 10 μM U73343 with 200 μM SA (A), or the PLD inhibitor 0.5% 1-butanol or 0.5% Tert-butanol (Tert) with 200 μM SA (B). In all treatment groups, cells were pre-incubated with the inhibitors for 15 min before the 30 min SA treatment. The SA level in the cells after the different treatments was evaluated by HPLC (see Materials and Methods). The data represent the mean of 3 independent experiments ± SE, *P < 0.001