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. 2014 Mar 17;3:e28269. doi: 10.4161/onci.28269

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Copyright © 2014 Landes Bioscience

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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graphic file with name onci-3-e28269-g1.jpg — Figure 1. Preclinical model unveiling the activity of cytokine-induced cells against autologous bone sarcoma and soft tissue sarcoma. Primary sarcoma cultures were generated from fresh surgical biopsies. Putative cancer stem cells (CSCs) were visualized upon the transduction of malignant cells with a lentiviral CSC-detecting vector encoding the enhanced variant of green fluorescent protein (eGFP) under the control of the OCT4 promoter (pRRL.sin.PPT.hOct4.eGFP.Wpre). Patient-derived cytokine-induced killer (CIK) cells were active in vitro and in vivo against autologous bone sarcoma (BS) and soft tissue sarcoma (STS) cells, including putative CSCs.