Table 1. List of specific proteins identified by GFP-D-HURP AP-MS.

Protein Name	% Coverage	No. Peptides	MW [kDa]	Score
D-HURP (Mars)	72.20	77	101.9	1378.32
Fs(2)Ketel (Importin β)*	63.12	52	98.6	1372.07
Karybeta3 (Importin β)*	61.36	61	123.5	1192.46
PP1A-96A	33.64	10	37.3	75.78
Ensconsin	18.73	7	92.0	51.34
Dgt6	21.25	10	72.8	48.34
CG2017	16.19	7	75.1	43.86
Dgt5	15.33	9	77.9	37.81
Dgt3	20.71	8	65.8	31.68
ArfGAP3 *	17.93	5	54.4	31.13

GFP-D-HURP embryo extracts were incubated with GFP-TRAP-A beads, washed and subjected to Trypsin digestion, LC-MSMS and database searches. The proteins in Table 1 constitute those that were: (i) either identified in negative controls with scores of at least 4-fold less than in GFP-D-HURP (asterisks) or not identified in negative controls and (ii) had an MS score of > 30 and (iii) had > 10% coverage (% Coverage represents the % of the protein sequence covered by the peptides identified by LC-MSMS). The Importin-β proteins, Fs(2)Ketel and Karybeta3, are found at ~7 and 5-fold greater amounts respectively in GFP-D-HURP AP-MS, in relation to control experiments. Augmin subunits Dgt6, Dgt5 and Dgt3 were not present in the negative controls.