Figure 2. Effects of trehalose on motor neuron survival in SOD1G93A mice. (A) Representative photomicrographs of motor neurons in the anterior horn of spinal cords of 4 mouse groups by Nissl staining; (C) Representative photomicrographs of motor neurons in the anterior horn of spinal cord of 4 mouse groups by SMI-32 immunostaining. There were 3 mice in each group; asterisks mark the motor neurons. Scale bar: 100 μm; (B) The number of motor neurons in L4-5 segments by Nissl staining; (D) The mean number of SMI-32 positive motor neurons in both sides of one slice of an L4-5 segment. Data were analyzed using one-way ANOVA followed by Tukey post hoc test. All values are presented as mean ± SEM ##P < 0.001 as compared with WT mice; **P < 0.01 as compared with Tg-NT mice; &&P < 0.01 as compared with Tg-Suc mice.