Figure 5. Effects of trehalose on the MTOR-independent pathway and autophagy-related proteins. Western blot analysis of protein levels of p-MTOR (A), p-AKT1 (B), and p-RPS6KB (C) in the 4 mouse groups (WT, Tg-NT, Tg-Suc, Tg-Tre). Quantitative analysis of the ratio of p-MTOR/MTOR (D), p-AKT1/AKT1 (E) and p-RPS6KB/RPS6KB (F) in the 4 mouse groups. There were 3 mice in each group; ^##P < 0.01 when compared with WT mice. Western blot analysis of protein levels of LC3-II (G) and ATG5 (H) in 4 mouse groups. Quantitative analysis of LC3-II/LC3-I (I) and ATG5 (J). There were 3 mice in each group; ^##P < 0.01 as compared with WT mice. (K) Immunostaining of LC3 in the motor neurons of 4 mouse groups. Scale bar: 20 μm; (L) Quantitative analysis of LC3 puncta numbers in the 4 different mouse groups. There were 3 mice in each group. Data were analyzed using one-way ANOVA followed by Tukey post hoc test. All values are presented as mean ± S.E.M, ^##P < 0.01 as compared with WT mice.