Figure 6. Effects of trehalose on SQSTM1 level and protein aggregation in SOD1G93A mice. (A) Double labeling of SQSTM1 and SOD1 in the motor neurons of WT and SOD1G93A mice. (D) Immunostaining of ubiquitin in motor neurons. Scale bar: 20 μm. Quantitative analysis of SQSTM1 density (B), SOD1 density (C) and ubiquitin density (E) in motor neurons. Western blot analysis of SQSTM1 level (F), soluble and insoluble SOD1 protein level (H) and high molecular weight bands of ubiquitinated proteins (J) in the 4 different mouse groups. Quantitative analysis of SQSTM1 protein levels (G), human soluble SOD1 and insoluble SOD1 protein (I) and ubiquitinated proteins levels (K) in the spinal cord of SOD1G93A mice. There were 3 mice in each group. Data were analyzed using one-way ANOVA followed by Tukey post hoc test. Values are presented as mean ± SEM ##P < 0.01 and #P < 0.05 as compared with WT mice; **P < 0.01 and *P < 0.05 as compared with Tg-NT mice; &&P < 0.01 and &P < 0.05 as compared with Tg-Suc mice.