Figure 6. The effect of aminochrome on SQSMT1 degradation. (A) The SQSMT1 accumulated in U373MGsiGST6 cells (AM, lane 6) treated with 75 µM aminochrome (AM) for 24 h, in contrast with U373MG WT (C, lane 1), U373MGpSR (C, lane 2), and U373MGsiGST6 C, (lane 3) cells incubated with cell culture medium (C) and also U373MG WT (AM, lane 4) and U373MGpSR (AM, lane 5) cells treated with 75 µM aminochrome for 24 h. The results from analysis of 4 immunoblots are plotted in (B). The statistical significance was assessed using analysis of variance (ANOVA) for multiple comparisons and Student t test (*P < 0.05; ***P < 0.001).