Figure 3. Far-UV CD spectra (protein solution minus buffer) of the yeast Atg3 proteins in 50 mM potassium buffer, pH 8.0. The spectra for Atg3 (A), Atg3FR (B), and Atg3∆FR (C) were measured at 25 °C, at 90 °C, and at 25 °C after heating to 90 °C (25 °C cooled). Each spectrum is the average of 15 scans. Experimental conditions were as follows: time constant, 1s; bandwidth, 1 nm; path length, 1 mm; sample volume, 300 μl.