Figure 5. Intrinsic disorder predictions. (A) PONDR scores from PONDR-FIT, PONDR-VSL2, and PONDR-VL3 predictors plotted as a function of residue position in the amino acid sequence of yeast Atg3, Atg3FR, Atg3∆FR, and human ATG3. (B) ∆CH-∆CDF analysis of the Atg3 variants. The coordinates of each protein represent a distance of the corresponding protein from the boundary in the CH plot and the CDF plot. Q1 contains proteins predicted to be disordered based on the CH plot, but ordered based on the CDF plot; the Q2 quadrant includes proteins predicted to be ordered by both CH and CDF plots; the Q3 quadrant contains proteins predicted to be ordered by the CH plot, but disordered by the CDF plot; the Q4 quadrant includes proteins predicted to be disordered by both the CH and CDF plots. The ∆CDF parameter was calculated from PONDR-VL3.