Figure 1. Andrographolide prevents colitis-associated tumorigenesis. Mice were injected i.p. with a single dose (7.5 mg/kg) of AOM followed by 3 cycles of 2.5% DSS given in the drinking water for 5 d. Andro (7.5 and 15 mg/kg) was given i.g. daily during the interval between DSS cycles as shown. Mice were sacrificed on d 95 after CAC induction. (A) Body weight was recorded. (B) The inside of the colon was photographed. (C) Colon tissues were fixed and stained with H&E. (D) Tumor numbers were counted. (E and F) Tumor diameter and distribution were measured. (G) The tumor load was determined by totaling the diameters of all tumors for a given animal. Values are mean ± SEM of 9 mice/group. *P < 0.05, **P < 0.01 vs. AOM+DSS group. Andro, andrographolide.