Figure 2. Andrographolide inhibits inflammation in a colitis-associated colorectal cancer model. Mice were subjected to the AOM-DSS model. For other details, see the legend of Figure 1. (A) The expression of PCNA, p-STAT3, p-RELA/p-p65, and PTGS2/COX2 were analyzed by immunochemistry in paraffin-embedded colon sections. Data shown are representative of 3 experiments. (B) The expressions of PCNA, p-STAT3, p-RELA, and PTGS2 in colonic tissues were examined by western blotting. (C) Statistical data of the expressions of protein from 3 mice were shown. (D and E) The mRNA expressions of Hif1a, Vegfa, Tnf, Il17a, Il6, and Ptgs2 in colon sections were determined by real-time PCR. Data are presented as means ± SEM (n = 6). *P < 0.05, **P < 0.01 vs. AOM+DSS group. Andro, andrographolide.