Figure 4. Andrographolide inhibits NLRP3 inflammasome activation in mice with DSS-induced colitis. Mice were treated with 2.5% DSS in their drinking water for 7 d to induce acute colitis. Andro was administered daily via i.p. injection. (A) Peritoneal macrophages from mice were isolated on d 7. After stimulation with 5 mM ATP for 30 min, proteins were collected for western blotting. *P < 0.05. (B) Sections of colonic tissues were immunostained with DAPI (blue) and anti-ITGAM/CD11b-FITC (green) and were observed by fluorescence microscopy. Scale bar: 100 μm. Data shown are representative of 3 experiments. Andro, andrographolide.